
Typical Drosophila vials
Housing
Flies are typically kept in plastic or glass containers. I strongly recommend polypropylene plastic vials. Glass containers seem to suffer from condensation to a much greater extent. This can prevent the media from adhering to the vial (allowing it to slip when the vial is inverted), and prevent flies from climbing up the side of the vial.
Re-useability is something that we should all aim for, however fruit fly vials get SO 'glued-on' that it is often very messy and time consuming to clean them effectively, and fly keepers often use disposable cups. However, polypropylene plastic vials with straight sides really are the best choice. The media stays in place better, and if soaked and washed with a bottle brush, can be cleaned and reused easily. Conical flasks are just that bit harder to clean. Such containers are somewhat hard to source, but are available from scientific suppliers online.
Vials can be sealed with sponge (you can make your own - though they are somewhat difficult to cut perfectly; but again available online from scientific suppliers), these are good at keeping out mites, and very convenient. Less good options include: cotton wool (cheap, easy, but only good for vials with small openings. Not reuseable.), filter paper (can be damaged when wet) or netting (cheap, reuseable, can allow mites and interbreeding with wild flies). If you use netting, it is best to keep the flies in a cabinet to keep out other flies.

A funnel is critical when transferring flies
Handling fruit flies?
Under laboratory conditions flies need to be moved from container to container without any escape. This is performed by anaesthetising the flies with CO2 or ether. This renders them unconscious for a minute or two without damage. Even fully flighted flies can be easily managed in this way. If you have a cylinder of CO2 handy, perhaps for a planted aquarium, try inverting the vial, and introducing a gentle flow of CO2 by inserting a fine tube past the sponge topper. After just a few seconds the flies will fall unconscious. This is where the consistency of the media is essential. If your flies are kept over sloppy media, when it is inverted, media will slide down the side of the container, sticking to flies and potentially falling out. If you use the Sussex miedia (see fruit fly food recipes) it will stay exactly where it should! Tap the container a couple times, and all the sleeping flies will all fall out. No mess, just flies.
Luckily flightless flies are relatively easy to manipulate without CO2, as long as you work quickly. If you have difficulties, 5-8 minutes in a freezer greatly reduces their mobility. But work quickly, because they will wake up soon!
Always use funnels when moving flies from one vial to another. This will largely prevent escape, and allows a vial to be left 'unlidded' with flies still in it. If any do start to walk back up, a gentle tap soon sends them back to their food!
Fruit fly cultures for sale
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Dover, Pennsylvania, 173**, UNITED STATES OF AMERICA
$29
32 oz Drosophila Melanogaster (4 Pack) "Turkish Glider" Fruit Fly Culture
Our Fruit Flies are made in house with Repashy
Turkish Glider Melanogaster are the smallest size of fruit flies at 1 / 16 "
Turkish Glider Melanogaster are a winged and slightly darker than the Golden Wingless Melanogaster
This winged form of melanogaster can 'glide' short distances making for a more active feeder enticing even the pickiest of eaters
Turkish Gliders produce fast and consistently with ...
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Baldwin Park, California, UNITED STATES OF AMERICA
$8
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Dubuque, Iowa, 520**, UNITED STATES OF AMERICA
$8
Looking for an easy and reliable feeder insect for your dart frogs, fish, mantids, or juvenile reptiles? Our D. melanogaster Fruit Fly Cultures are the perfect choice! These small, flightless fruit flies can be easily produced at home offering a continuous supply for 30-50 days!
Product Features:
Species: Drosophila melanogaster (D. melanogaster) Fruit FliesPerfect for: Dart frogs, fish, mantids, juvenile reptiles, and other insectivoresCulture Lifespan: Each culture typically produces ...
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Cleveland, Ohio, 441**, UNITED STATES OF AMERICA
$30
Flightless Fruit Fly Cultures (40-50 flies included per cup!)
WE ARE ONLY ABLE TO OFFER LIVE ARRIVAL GUARNTEE WITH ORDERS SHIPPED VIA FEDEX 2DAY!
Flightless fruit flies (Drosophila hydei) are about ???? and are a great feeder for small lizards, spiders, mantids and frogs. Each culture will arrive with at least 40-50 live fruit flies
These are producing cultures that are about 2 weeks old. They will continue producing new flies for another two to three weeks. These are flightless fruit ...
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Dover, Pennsylvania, 173**, UNITED STATES OF AMERICA
$22
This 2 pack of Flightless Fruit Fly cultures is the perfect addition to any reptile or dart frog owner's collection. These cultures provide a nutritious and tasty food source for your pets without the hassle of dealing with live insects. The flies are easy to maintain and will last for several weeks, ensuring that your pets are well-fed and healthy.
Whether you're a seasoned reptile owner or a new dart frog enthusiast, these Flightless Fruit Fly cultures are sure to be a hit with your pets....
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