Typical Drosophila vials. Keeping flies over damp paper in a dedicated cabinet helps maintain humidity, and prevents contamination from wild flies
Typical Drosophila vials. Keeping flies over damp paper in a dedicated cabinet helps maintain humidity, and prevents contamination from wild flies.

Why a regime?

Looking after Drosophila is quite easy when you get the hang of it. When cultures crash it's usually because they were left unattended for too long. The life span (just like the development time) is temperature dependent, and does not much exceed 2 weeks as a fly. It is critical to follow a careful regime to allow maximum production. This can take a few minutes each week and that's it. HOWEVER, miss a week and your yield will be drop, miss 2 and you may well loose your cultures! If flies are left too long over old media, they will also starve, and provide a poor snack for the fish. If you can not attend to the flies, its best to keep them cool, slowing down their life cycle (20 deg C) until you can tend to them again.

In general flies should be kept at around 25-28 degrees C. This provides a quick turnover of flies for feeding and allows a weekly regime to be followed. They also like a relatively high humidity; If yours are not kept in a humid room, consider keeping the vials in half a centimeter of water.

So how does the regime work?

The vials that the flies inhabit are separated into 3 categories:

  1. The present generation of adult flies - laying eggs for future generations (3-4 vials - top shelf of picture)
  2. Vials containing chrysalised larvae, and newly emerged flies. (10-20 vials - middle shelf of picture)
  3. Flies or larvae for feeding fish (3-4 vials - bottom shelf of picture).

Once per week newly emerged flies are collected from 2. These are placed in fresh vials, and are designated 1, the present generation. After 3-4 days these flies are moved again, onto new media 3. The old vials (which are loaded with eggs or very young larvae) go back in 2, to make future generations. Once the week has passed, the flies making the present generation can be fed to your fish, and their vials may either go back into 2, or be left for a few days to provide larvae for feeding (see part 5 - feeding fish). Of course a new generation of adult flies is again collected from 2 at the same time.

In hot weather, and if things are going well, you will soon have a lot of flies. On the other hand, you may struggle to breed enough to satisfy your fish. But always avoid feeding newly emerged flies to your fish, even if you have no shortage of flies 'coming through'. Newly collected flies may well be hungry. It's always a good idea to place them over fresh media with added brewers yeast for 24 hours before feeding them to fish, ensuring they are plump and healthy before feeding to your killies.

There are many advantages of keeping to a strict regime such as this:

  • Each vial will contain only flies/larvae of approximately the same age, resulting in eclosion (hatching of the adult fly) over a relatively short period. This reduces the time you have to hang onto older vials-making fly collection easier and lessening the chances of mold.
  • The longer flies are kept on the same media, the more runny it becomes- if the flies are collected after a 3-4 days, it will still be quite solid. Runny media can drown flies, stick them down and goes everywhere when the jar is inverted.
  • All flies will have reached sexual maturity and have reproduced before being fed.

Fruit fly cultures for sale

New BLACK Hydei 32 oz Fruit Fly Culture *HEAT PACK option*

Cordele, Georgia, 310**, UNITED STATES OF AMERICA


Due to winter months and cold temps, live arrival is voided without purchase of a heat pack! please be aware of this when purchasing. Live arrival voided during extreme temps. HEAT PACK OPTION AVAILABLE IF YOUR TEMPS WILL BE HIGH
Will have excelsior for surface area
DISCLAIMER: as we enter winter temps, there is a risk that cultures will not arrive alive or producing, because of this I have included a heat pack option. I will also be shipping priority VS first class so prices have ... more

Reptanicals Fruit Fly Feast Premium Fly Food for lasting cultures FREE SHIPPING

Clearlake Oaks, California, 954**, UNITED STATES OF AMERICA


Naturally enhance colors in reptiles!
Each resealable bag contains enough food to make 5x 32oz cultures!
Reptanicals Fruit Fly Feast provides optimal nutrition for the entire fly life cycle. Not only is our food free of chemicals and artificial mold inhibitors, it contains essential vitamins and minerals along with natural plant-based color enhancers. This nutrient packed food has been specially formulated for optimal gut-loading of feeder flies for healthy, vibrant reptiles with vibrant ... more

Flightless Fruit Fly Culture 32oz Live

Middletown, Pennsylvania, 170**, UNITED STATES OF AMERICA


Our Flightless Fruit Fly Culture 32 oz are made with Repashy
Live Flightless Fruit Flies are a great option for feeding small lizards and many species of frogs, especially because of their reproduction cycle. Each culture of flightless fruit flies has a lifespan of about one month, and even after they die, they will continually pupate from eggs laid by the adults in the culture! This will allow you to keep fruit flies for a while, as long as they are taken care of accurately
Oue Fruit ... more

Fruit Fly Traps STEM 8 traps 5 4 oz each

Oklahoma City, Oklahoma, 731**, UNITED STATES OF AMERICA


Fruit Fly Traps STEM 8 traps 5 4 oz each Safe for use around Kids and pets
You will receive 1 sealed case with 8 X 5 4 ox traps
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Fruit Fly Culture 4Pack Variety Live Flightless Turkish Gliders Golden and Hydei

Dover, Pennsylvania, 173**, UNITED STATES OF AMERICA


This Fruit Fly Culture 4Pack Variety is the perfect food for your pet reptiles. You'll receive live flightless fruit flies of different species, including Turkish Gliders, Golden, and Hydei. These fruit flies are bred by Crazy Frog Lady L L.C , a trusted brand in the pet supplies industry.
Use this variety pack to provide nutrition to your reptiles and keep them healthy. These live fruit flies are a great source of protein and are easy to digest. They also provide entertainment for your pets... more